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PLEK Antibody

CAT: 0013-GTR18124248Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124248Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PLEK Antibody
Product Name Alternative
PLEK, pleckstrin, P47, p47, FLJ27168
UniProt
P08567
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-EWIKAIQMASRTGK
Target
PLEK
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40-45kDa band observed in Human Parathyroid gland lysates and also in preliminary testing of Mouse Spleen lysates and lysates of cell line U937 (calculated MW of 40.1kDa according to Human NP_002655.2) . Recommended concentration: 0.01-0.3μg/mL. Primary incubation 1 hour at room temperature. Negative Control: HepG2 (hepatocellular carcinoma) cell lysate.Immunohistochemistry:Paraffin embedded Human Vessel (platelets) and Spleen. Recommended concentration: 3.75μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_002655.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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CSB-PA018152GA01HUPLEK Antibody

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