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RPL22 Antibody

CAT: 0013-GTR18124173Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124173Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RPL22 Antibody
Product Name Alternative
RPL22, ribosomal protein L22, EAP, HBP15, HBP15/L22, 60S ribosomal protein L22, EBER-associated protein, Epstein-Barr virus small RNA-associated protein, Epstein-Barr-encoded RNA-associated protein, heparin-binding protein 15, heparin-binding protein HBp15
UniProt
P35268
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-SKESYELRYFQINQ
Target
RPL22
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 17-18kDa and 25kDa in Human Liver lysates after 0.5μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8kDa according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . This batch is not expected to cross-react with Mouse and Rat in Western blot.Immunohistochemistry:In paraffin embedded Human Brain (Cortex) shows cytoplasm staining in neuronal bodies. Recommended concentration: 3-6μg/mL. Paraffin embedded Human Thyroid, Uterus and Testis. Recommended concentration: 3.75μg/mL.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_000974.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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