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SEMA3E Antibody

CAT: 0013-GTR18124128Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124128Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SEMA3E Antibody
Product Name Alternative
SEMA3E, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E, KIAA0331, M-SEMAH, M-SemaK, M-sema H, SEMAH, coll-5 , sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E, semaphorin 3E
UniProt
O15041
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-NGGRYGTTKDYPDD
Target
SEMA3E
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6μg/mL.
Prediction Reactivity
Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_036563.1, NP_001171600.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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