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SIPA1 Antibody

CAT: 0013-GTR18124109Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124109Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SIPA1 Antibody
Product Name Alternative
SIPA1, SPA1, MGC17037, signal-induced proliferation-associated gene 1, p130 SPA-1, GTPase-activating protein Spa-1, signal-induced proliferation-associated 1, MGC102688, signal-induced proliferation-associated gene 1, signal-induced proliferation-associated protein 1
UniProt
Q96FS4
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-ASKQLGSPTADLA
Target
SIPA1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272 (44) :28081-8; PMID: 9346962) . Recommended concentration: 1-3μg/mL. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_694985.28, NP_006738.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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