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ASS1 Antibody

CAT: 0013-GTR18123896Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18123896Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ASS1 Antibody
Product Name Alternative
Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase
UniProt
P00966
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for this antibody is: C-ENPKNQAPPGLYTKTQD
Target
ASS1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6kDa according to Mouse NP_031520.1) . Recommended concentration: 0.03-1μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_000041.2, NP_446464.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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