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APOBEC3A Antibody

CAT: 0013-GTR18123820Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18123820Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
APOBEC3A Antibody
Product Name Alternative
APOBEC3A, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, ARP3, PHRBN, bK150C2.1, phorbolin 1
UniProt
P31941
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-TSNFNNGIGRHKTY
Target
APOBEC3A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 26-28kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0kDa according to Human NP_663745.1) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-1μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Recommended concentration: 3-6μg/mL.Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10μg/mL.
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_663745.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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