Products for Research Use Only

ANK1 Antibody

CAT: 0013-GTR18123793Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18123793Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ANK1 Antibody
Product Name Alternative
ANK1 , ankyrin 1, erythrocytic , ANK, SPH1, SPH2, ankyrin 1, ankyrin-1, erythrocytic, ankyrin-R
UniProt
P16157
Immunogen
The immunogen for this antibody is: C-QIVKRASLKRGKQ
Target
ANK1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kDa according to NP_065211.2. The 19-22 kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Bovine, Human, Mouse, Rat
Tested Applications
ELISA
NCBI Accession Number
NP_065208.2, NP_065213.2, NP_065209.2, NP_065210.2, NP_000028.3, NP_065211.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.