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GABRB3 Antibody

CAT: 0013-GTR18123739Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18123739Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GABRB3 Antibody
Product Name Alternative
GABRB3, gamma-aminobutyric acid (GABA) A receptor, beta 3, MGC9051, GABA (A) receptor beta-3 subunit, GABA-A receptor beta-3 subunit, GABA-alpha receptor beta-2 subunit, GABAA receptor beta-3 subunit
UniProt
P28472
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-EKTAKAKNDRSK
Target
GABRB3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments in Human Brain (Cerebral Cortex, Frontal Cortex and Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1μg/mL) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Cortex shows patchy staining near or at the neuronal cell membrane.. Recommended concentration, 2-4μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_000805.1, NP_068712.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · P28472

Gamma-aminobutyric acid receptor subunit beta-3

GBRB3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P28472
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GABRB3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B7Z2W1, B7Z825, F5H3D2, H7BYV8, Q14352, Q96FM5
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCytoplasmic vesicleMembranePostsynaptic cell membraneSynapse

Ligand

Chloride

Molecular function

Chloride channelIon channelLigand-gated ion channelReceptor

Disease

Disease variantEpilepsy

PTM

Disulfide bondGlycoprotein

Biological process

Ion transportTransport

Domain

SignalTransmembraneTransmembrane helix

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