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FZD2 Antibody

CAT: 0013-GTR18123675Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18123675Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
FZD2 Antibody
Product Name Alternative
FZD2 , frizzled homolog 2 (Drosophila), frizzled 2
UniProt
Q14332
Reactivity
Human
Immunogen
The immunogen for this antibody is: RYATLEHPFHCPR
Target
FZD2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60-65kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24kDa was also observed in some lysates which was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.03-0.1μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5μg/mL.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_001457.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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