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PENK Antibody

CAT: 0013-GTR18123633Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18123633Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PENK Antibody
Product Name Alternative
Proenkephalin
UniProt
P22005
Reactivity
Mouse, Rat
Immunogen
The immunogen for this antibody is: C-YKDSSKQDESH
Target
PENK
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa according to Mouse NP_001002927.1 and 30.9kDa according to Rat NP_058835.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus. Recommended concentration, 0.02-0.05μg/mL.
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_001002927.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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