Multiplex ELISA Kit For Porcine Cytokine Array (4-plex)

• Description:

  Product Overview

  The Multiplex Porcine Cytokine – High Sensitivity (4-plex) is a fully quantitative, ELISA-based chemiluminescent assay allowing concurrent measurement of cytokines in serum or plasma samples.

  A kit contains a 96-well plate, with each well featuring the 4-plex along with a positive control with all the other reagents necessary to perform the test. Our high-quality reagents help ensure the accuracy of your results.

  Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 4.83 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

  Assay Ranges

  Analytes	Calibrator Range	Units	LLD
  IL-1β	1550 - 2.13	pg/mL	2.1
  IL-6	1800 - 2.47	pg/mL	2.5
  IL-8	2100 - 2.88	pg/mL	2.9
  TNFα	1600 - 2.19	pg/mL	1.9

  Kit Components

  Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

  Reagents Include:

  Calibrator: Lyophilized, recombinant antigens in a buffered protein base
  Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
  Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
  Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
  Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with blockers and preservatives
  Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
  Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
  Plate Seals (3): Adhesive strips

• CAS Number:

  7732-18-5