RFWD2 (Phospho-Ser387) Colorimetric Cell-Based ELISA Kit

• Description:

  The RFWD2 (Phospho-Ser387) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RFWD2 (Phospho-Ser387) protein expression profile in cells. The kit can be used for measuring the relative amounts of RFWD2 (Phospho-Ser387) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RFWD2 (Phospho-Ser387) .

• Synonyms:

  E3 ubiquitin-protein ligase COP1 ;2.3.2.27 ; Constitutive photomorphogenesis protein 1 homolog ; hCOP1 ; RING finger and WD repeat domain protein 2 ; RING finger protein 200; RING-type E3 ubiquitin transferase RFWD2 ; COP1 ; RFWD2 , RNF200

• Gene Name:

  COP1 RFWD2 , RNF200

• UniProt:

  Q8NHY2

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable) .

• Molecular Weight:

  80474 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  E3 ubiquitin-protein ligase COP1

• Subcellular Location:

  Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.