Cortactin (Phospho-Tyr466) Colorimetric Cell-Based ELISA Kit

• Description:

  The Cortactin (Phospho-Tyr466) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Cortactin (Phospho-Tyr466) protein expression profile in cells. The kit can be used for measuring the relative amounts of Cortactin (Phospho-Tyr466) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Cortactin (Phospho-Tyr466) .

• Synonyms:

  Src substrate cortactin; Cttn; Ems1

• Gene Name:

  Cttn

• UniProt:

  Q60598

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed in most tissues, except in B- lymphocytes or plasma cells.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:17403031) . Plays a role in the formation of lamellipodia and in cell migration (By similarity) . Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity) . Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (PubMed:22262902) . Plays a role in the invasiveness of cancer cells, and the formation of metastases (By similarity) . Plays a role in focal adhesion assembly and turnover (By similarity) . In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1- phosphate (S1P) -mediated endothelial cell (EC) barrier enhancement (By similarity) . Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782) . Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity) . Required for stabilization of KCNH1 channels at the cell membrane (By similarity) .

• Molecular Weight:

  61250 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Src substrate cortactin

• Subcellular Location:

  Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cytoplasm, cell cortex. Cell projection. Cell projection, podosome. Cell projection, dendritic spine. Cell projection, dendrite. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Membrane, clathrin-coated pit. Cell junction, focal adhesion. Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity) . Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers. In the presence of CTTNBP2, localizes at the cell cortex. In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft. Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity) .