4E-BP1 (Phospho-Thr36) Colorimetric Cell-Based ELISA Kit

• Description:

  The 4E-BP1 (Phospho-Thr36) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor 4E-BP1 (Phospho-Thr36) protein expression profile in cells. The kit can be used for measuring the relative amounts of 4E-BP1 (Phospho-Thr36) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on 4E-BP1 (Phospho-Thr36) .

• Synonyms:

  Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I; EIF4EBP1

• Gene Name:

  EIF4EBP1

• UniProt:

  Q13541

• Reactivity:

  Human, Mouse, Rat

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

• Molecular Weight:

  12580 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Eukaryotic translation initiation factor 4E-binding protein 1