ZAP-70 (Phospho-Tyr292) Colorimetric Cell-Based ELISA Kit

• Description:

  The ZAP-70 (Phospho-Tyr292) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ZAP-70 (Phospho-Tyr292) protein expression profile in cells. The kit can be used for measuring the relative amounts of ZAP-70 (Phospho-Tyr292) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ZAP-70 (Phospho-Tyr292) .

• Synonyms:

  70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70; ZAP70; kinase ZAP70

• Gene Name:

  ZAP70

• UniProt:

  P43403

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative) . Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR) .

• Molecular Weight:

  69872 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Tyrosine-protein kinase ZAP-70

• Subcellular Location:

  Cytoplasm. Cell membrane; Peripheral membrane protein. In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity) .