WASP (Phospho-Tyr290) Colorimetric Cell-Based ELISA Kit

• Description:

  The WASP (Phospho-Tyr290) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor WASP (Phospho-Tyr290) protein expression profile in cells. The kit can be used for measuring the relative amounts of WASP (Phospho-Tyr290) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on WASP (Phospho-Tyr290) .

• Synonyms:

  IMD2; WAS, Wiskott-Aldrich syndrome protein; Wiskott-Aldrich syndrome protein homolog

• Gene Name:

  WAS

• UniProt:

  P42768

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed:12235133, PubMed:12769847, PubMed:16275905) . Important for efficient actin polymerization (PubMed:8625410, PubMed:12235133, PubMed:16275905) . Possible regulator of lymphocyte and platelet function (PubMed:9405671) . Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria (PubMed:18650809) . In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:20574068) . Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947) .

• Molecular Weight:

  52913 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Wiskott-Aldrich syndrome protein

• Subcellular Location:

  Cytoplasm, cytoskeleton. Nucleus.