LAT (Phospho-Tyr191) Colorimetric Cell-Based ELISA Kit

• Description:

  The LAT (Phospho-Tyr191) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor LAT (Phospho-Tyr191) protein expression profile in cells. The kit can be used for measuring the relative amounts of LAT (Phospho-Tyr191) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on LAT (Phospho-Tyr191) .

• Synonyms:

  36 kDa phospho-tyrosine adaptor protein; Linker for activation of T cells; p36-38; pp36

• Gene Name:

  LAT

• UniProt:

  O43561

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level) .

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Required for TCR (T-cell antigen receptor) - and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III) -mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor) -mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

• Molecular Weight:

  27930 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Linker for activation of T-cells family member 1

• Subcellular Location:

  Cell membrane; Single-pass type III membrane protein. Present in lipid rafts.