VASP (Phospho-Ser157) Colorimetric Cell-Based ELISA Kit

• Description:

  The VASP (Phospho-Ser157) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor VASP (Phospho-Ser157) protein expression profile in cells. The kit can be used for measuring the relative amounts of VASP (Phospho-Ser157) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on VASP (Phospho-Ser157) .

• Synonyms:

  Vasodilator-stimulated phosphoprotein; VASP

• Gene Name:

  VASP

• UniProt:

  P50552

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Highly expressed in platelets.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin- bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

• Molecular Weight:

  39830 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Vasodilator-stimulated phosphoprotein

• Subcellular Location:

  Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes) .