Keratin 14 Colorimetric Cell-Based ELISA

• Description:

  The Keratin 14 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Keratin 14 protein expression profile in cells. The kit can be used for measuring the relative amounts of Keratin 14 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Keratin 14.

• Synonyms:

  Keratin, type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14; KRT14

• Gene Name:

  KRT14

• UniProt:

  P02533

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

• Molecular Weight:

  51561 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Keratin, type I cytoskeletal 14

• Subcellular Location:

  Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.