SMC1 Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1531Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No

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CAT#:0519-EKC1531Size:1 Kit, containing one 96 Well Plate and all necessary reagents

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

The SMC1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SMC1 protein expression profile in cells. The kit can be used for measuring the relative amounts of SMC1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SMC1.

Synonyms

Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb1.8; SMC1A; DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1

Gene Name

SMC1A

UniProt

Q14683

Reactivity

Human, Mouse

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Molecular Weight

143233 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Structural maintenance of chromosomes protein 1A

Subcellular Location

Nucleus. Chromosome. Chromosome, centromere, kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere- kinetochore complex at the kinetochore region during mitosis.

UniProtKB · Q14683

Structural maintenance of chromosomes protein 1A

SMC1A_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q14683
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: SMC1A
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: SMC protein 1A, SMC-1-alpha, SMC-1A
EC number: —
Processing: —
Secondary accessions: O14995, Q16351, Q2M228

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Ligand

ATP-bindingNucleotide-binding

Biological process

Cell cycleCell divisionDNA damageDNA repairMeiosisMitosis

Cellular component

CentromereChromosomeKinetochoreNucleus

Domain

Coiled coil

Disease

Disease variantEpilepsyIntellectual disability

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MBS9500554-01	SMC1 Colorimetric Cell-Based ELISA Kit
OKAG01043	SMC1 Colorimetric Cell-Based ELISA Kit (OKAG01043)
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