PI3-kinase p85-alpha/gamma Colorimetric Cell-Based ELISA Kit

• Description:

  The PI3-kinase p85-alpha/gamma Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PI3-kinase p85-alpha/gamma protein expression profile in cells. The kit can be used for measuring the relative amounts of PI3-kinase p85-alpha/gamma in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PI3-kinase p85-alpha/gamma.

• Synonyms:

  Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R1; GRB1

• Gene Name:

  PIK3R1

• UniProt:

  P27986

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level) .

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429) . Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923) .

• Molecular Weight:

  83598 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Phosphatidylinositol 3-kinase regulatory subunit alpha