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EGFR Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1185Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1185Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The EGFR Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor EGFR protein expression profile in cells. The kit can be used for measuring the relative amounts of EGFR in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on EGFR.
Synonyms
Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB1
Gene Name
EGFR
UniProt
P00533
Reactivity
Human, Mouse, Rat
Tissue Specificity
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity) .
Molecular Weight
134277 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Epidermal growth factor receptor
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) .

UniProtKB · P00533

Epidermal growth factor receptor

EGFR_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P00533
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EGFR
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.10.1
Processing
Precursor
Secondary accessions
O00688, O00732, P06268, Q14225, Q68GS5, Q92795, Q9BZS2, Q9GZX1, Q9H2C9, Q9H3C9, Q9UMD7, Q9UMD8, Q9UMG5
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell membraneEndoplasmic reticulumEndosomeGolgi apparatusMembraneNucleusSecreted

Molecular function

Developmental proteinHost cell receptor for virus entryKinaseReceptorTransferaseTyrosine-protein kinase

Disease

Disease variantProto-oncogene

PTM

Disulfide bondGlycoproteinHydroxylationIsopeptide bondLipoproteinMethylationPalmitatePhosphoproteinUbl conjugation

Biological process

Host-virus interaction

Domain

RepeatSignalTransmembraneTransmembrane helix

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