Anti-Phospho-MEK1 (Thr386) MAP2K1 Antibody

• Background:

  MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003) . This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002) . Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) . Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr-292 and Thr-386 (Mansour et al., 1994) .

• Description:

  Boster Bio Anti-Phospho-MEK1 (Thr386) MAP2K1 Antibody (Catalog # P00292-2) . Tested in WB applications. This antibody reacts with Human, Rat.

• Phospho Site:

  Phosphorylated, Thr386

• Synonyms:

  Dual specificity mitogen activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, ERK activator kinase 1 antibody, MAP kinase kinase 1 antibody, MAP kinase/Erk kinase 1 antibody, MAP2K1 antibody, MAPK/ERK kinase 1 antibody, MAPKK 1 antibody, MAPKK1 antibody, MEK 1 antibody, Mek1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, MKK 1 antibody, MKK1 antibody, MP2K1_HUMAN antibody, PRKMK1 antibody, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody, Protein kinase mitogen activated kinase 1 antibody

• Gene Name:

  MAP2K1

• UniProt:

  Q02750

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 of human MEK1, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Human.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widely expressed, with extremely low levels in brain.

• Applications:

  WB

• Field of Research:

  MAPK Pathway, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

• Purification:

  Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Dilution:

  WB: 1:1000

• Form:

  Liquid

• Function:

  Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

• Molecular Weight:

  43439 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

• Fragment:

  IgG

• Applications Notes:

  Specific for endogenous levels of the ~45 kDa MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

• Other Gene Names:

  Dual specificity mitogen-activated protein kinase kinase 1

• Subcellular Location:

  Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.

• Prediction Reactivity:

  Bovine, Canine, Chicken, Primate, Mouse, Xenopus

• Protein Name:

  Dual specificity mitogen-activated protein kinase kinase 1