Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248

• Description:

  Boster Bio Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248 (Catalog # M04635-1) . Tested in WB, ICC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.

• Synonyms:

  Core histone macro-H2A.1; Histone macroH2A1; mH2A1; H2A.y; H2A/y; H2afy

• Gene Name:

  H2afy

• UniProt:

  Q9QZQ8

• Host:

  Rabbit

• Reactivity:

  Human, Vertebrates

• Cross Reactivity:

  This antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross-reactivity with other histone proteins.

• Immunogen:

  A peptide corresponding to the C-terminus of human Histone macroH2A1.

• Clonality:

  Monoclonal

• Clone:

  Clone: RM248

• Tissue Specificity:

  Widely expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level) . In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, expressed in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla.

• Applications:

  WB, ICC, ELISA, Luminex

• Field of Research:

  2339, Epigenetics and Nuclear Signaling, Histones

• Purification:

  Protein A affinity purified from an animal origin-free culture supernatant

• Concentration:

  1 mg/mL

• Form:

  Liquid

• Function:

  Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes (By similarity) . Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin (By similarity) (PubMed:16107708) .

• Molecular Weight:

  39735 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Other Gene Names:

  Core histone macro-H2A.1

• Subcellular Location:

  Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin (PubMed:9634239) . In quiescent lymphocytes, associated with centromeric constitutive heterochromatin (PubMed:15564378) .