Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody catalog # M03598. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  ATP synthase subunit alpha, mitochondrial; ATP5A1; ATP5A, ATP5AL2, ATPM

• Gene Name:

  ATP synthase subunit alpha, mitochondrial

• UniProt:

  P25705

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human ATP5A1

• Clonality:

  Monoclonal

• Clone:

  Clone: AAFC-1

• Tissue Specificity:

  Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. .

• Applications:

  WB, IHC, ICC, IF, Flow Cytometry

• Field of Research:

  ATPases, Cancer, Metabolism, Mitochondria, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Organelles, Oxidative Phosphorylation, Pathways and Processes, Plasma Membrane, Signal Transduction, Subcellular Markers, Tags & Cell Markers

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Mitochondrial membrane ATP synthase (F (1) F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F (1) . Rotation of the central stalk against the surrounding alpha (3) beta (3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity) . .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  FC 1:50

• Other Gene Names:

  ATP5A1

• Subcellular Location:

  Mitochondrion inner membrane . Cell membrane; Peripheral membrane protein; Extracellular side . Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951) .

• Protein Name:

  T-cell surface glycoprotein CD3 zeta chain