Anti-K63-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody

Anti-K63-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody

• Description: Boster Bio Anti-K63-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody catalog # M02848-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
• Synonyms: Polyubiquitin-B;Ubiquitin;UBB;
• CAS Number: 9007-83-4
• Gene Name: Polyubiquitin-B
• UniProt: P0CG47
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Immunogen: A synthesized peptide derived from human K63-linkage Specific Ubiquitin
• Clonality: Monoclonal
• Clone: Clone: IGC-21
• Applications: WB,IHC,ICC,IF,Flow Cytometry
• Purification: Affinity-chromatography
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Function: Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. .
• Storage Conditions: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 25762 MW
• Observed Molecular Weight: 55 kDa
• Fragment: Rabbit IgG
• Applications Notes: WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  FC 1:50