Anti-UBE2I/Ubc9 Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-UBE2I/Ubc9 Rabbit Monoclonal Antibody catalog # M02295. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  SUMO-conjugating enzyme UBC9;6.3.2.-; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18; UBE2I; UBC9, UBCE9

• Gene Name:

  SUMO-conjugating enzyme UBC9

• UniProt:

  P63279

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human UBE2I

• Clonality:

  Monoclonal

• Clone:

  Clone: EDF-21

• Tissue Specificity:

  Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. .

• Applications:

  WB, IHC, ICC, IF, IP, Flow Cytometry

• Field of Research:

  Metabolism, Obesity, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin & Ubiquitin Like Modifiers

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  IP 1:50
  FC 1:50

• Other Gene Names:

  UBE2I

• Subcellular Location:

  Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity) . .

• Protein Name:

  Lysosome-associated membrane glycoprotein 2 (Lamp2)