Anti-Desmoglein 2 Monoclonal Antibody

• Description:

  Boster Bio Anti-Desmoglein 2 Monoclonal Antibody catalog # M02035. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Melanoma antigen recognized by T-cells 1; MART-1; Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A; MLANA; MART1

• Gene Name:

  Melanoma antigen recognized by T-cells 1

• UniProt:

  Q14126

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human Desmoglein 2 Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

• Clonality:

  Monoclonal

• Clone:

  Clone: AFBF-4

• Tissue Specificity:

  Expression is restricted to melanoma and melanocyte cell lines and retina.

• Applications:

  WB, IHC, ICC, IF, Flow Cytometry

• Field of Research:

  Cadherins, Cancer, Cardiovascular, Cell Adhesion, Cytoskeleton/ECM, Invasion/Microenvironment, Signal Transduction

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:1000-1:5000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  FC 1:100

• Other Gene Names:

  DSG2

• Subcellular Location:

  Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.

• Protein Name:

  Lysosome-associated membrane glycoprotein 2 (Lamp2)