Anti-AIF AIFM1 Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-AIF AIFM1 Rabbit Monoclonal Antibody catalog # M01571. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Apoptosis-inducing factor 1, mitochondrial;1.1.1.-; Programmed cell death protein 8; AIFM1; AIF, PDCD8

• Gene Name:

  Apoptosis-inducing factor 1, mitochondrial

• UniProt:

  O95831

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human AIF

• Clonality:

  Monoclonal

• Clone:

  Clone: GH-1

• Tissue Specificity:

  Detected in muscle and skin fibroblasts (at protein level) . Isoform 5 is frequently down-regulated in human cancers. .

• Applications:

  WB, IHC, ICC, IF, IP, Flow Cytometry

• Field of Research:

  Apoptosis, Cancer, Cell Biology, Cell Cycle, Cell Death, Epigenetics and Nuclear Signaling, Invasion/Microenvironment, Metabolism, Metabolism Processes, Nuclear, Nucleus, Oxidative Stress, Pathways and Processes, Redox Metabolism

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:100-1:500
  ICC/IF 1:100-1:500
  IP 1:50
  FC 1:50

• Other Gene Names:

  AIFM1

• Subcellular Location:

  Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit) . During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol) . AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.

• Protein Name:

  Glycogen synthase kinase-3 beta (gsk3b)