Anti-Cytokeratin 14 KRT14 Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-Cytokeratin 14 KRT14 Rabbit Monoclonal Antibody catalog # M01432-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Keratin, type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14; KRT14

• Gene Name:

  Keratin, type I cytoskeletal 14

• UniProt:

  P02533

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human Cytokeratin 14

• Clonality:

  Monoclonal

• Clone:

  Clone: FEF-11

• Tissue Specificity:

  Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. .

• Applications:

  WB, IHC, ICC, IF

• Field of Research:

  Cancer,2339, Epigenetics and Nuclear Signaling, MAPK Pathway, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Transcription, Transcription Factors

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:1000-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200

• Other Gene Names:

  KRT14

• Subcellular Location:

  Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.

• Protein Name:

  Glycogen synthase kinase-3 beta (gsk3b)