Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody catalog # M01032. Tested in WB, ICC/IF applications. This antibody reacts with Human.

• Synonyms:

  Hepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Transcription factor 3B; TCF-3B; FOXA2; HNF3B, TCF3B

• Gene Name:

  Hepatocyte nuclear factor 3-beta

• UniProt:

  Q9Y261

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthesized peptide derived from human FOXA2

• Clonality:

  Monoclonal

• Clone:

  Clone: AAAH-6

• Tissue Specificity:

  Ubiquitous. .

• Applications:

  WB, ICC, IF

• Field of Research:

  Developmental Biology, Domain Families, Epigenetics and Nuclear Signaling, Forkhead Box, Lineage Markers, Lineage Specification, Metabolism, Obesity, Stem Cells, Transcription

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity) . In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation. .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:500-1:1000
  ICC/IF 1:50-1:200

• Other Gene Names:

  FOXA2

• Subcellular Location:

  Nucleus . Cytoplasm . Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.

• Protein Name:

  Glycogen synthase kinase-3 beta (gsk3b)