Anti-Myelin Basic Protein MBP Monoclonal Antibody

• Description:

  Boster Bio Anti-Myelin Basic Protein MBP Monoclonal Antibody catalog # M00211-2. Tested in IF, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.

• Synonyms:

  Myelin basic protein; MBP; Myelin A1 protein; Mbp; Shi

• Gene Name:

  Mbp

• UniProt:

  P04370

• Host:

  Mouse

• Reactivity:

  Bovine, Equine, Human, Mouse, Pig, Rat

• Immunogen:

  Purified myelin basic protein isolated from bovine brain

• Clonality:

  Monoclonal

• Tissue Specificity:

  In the embryo, isoform 1-isoform 3 are found in neurons within the central nervous system (primarily in pioneer neurons important in the formation of the cortex) and the peripheral nervous system. They are also expressed in the thymus, gut, lung and kidney. In the adult, isoform 1-isoform 3 are highly expressed in the brain (mainly in brain regions rich in oligodendrocytes) and spleen. Lower levels are seen in the heart, kidney and lung. Isoform 2 is also found in cells of the immune system. The isoforms missing the 134 first amino acids (isoform 4- isoform 13) are almost exclusively produced in the myelin-forming cells, the mature oligodendrocytes.

• Applications:

  IF, WB

• Field of Research:

  Tags & Cell Markers

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Dilution:

  WB: 1:5,000-1:10,000. IF/ICC and IHC: 1:1,000.

• Function:

  The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1- isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. .

• Molecular Weight:

  27168 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Other Gene Names:

  Myelin basic protein

• Subcellular Location:

  Isoform 13: Myelin membrane; Peripheral membrane protein; Cytoplasmic side.