Anti-CDK4 Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-CDK4 Rabbit Monoclonal Antibody catalog # M00159. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Cyclin-dependent kinase 4;2.7.11.22; Cell division protein kinase 4; PSK-J3; CDK4

• Gene Name:

  Cyclin-dependent kinase 4

• UniProt:

  P11802

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human CDK4

• Clonality:

  Monoclonal

• Clone:

  Clone: HOB-3

• Tissue Specificity:

  Expressed in testicular carcinoma and derived germ cell tumors (at protein level) . Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. .

• Applications:

  WB, ICC, IF, IP

• Field of Research:

  Cancer, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:1000-1:2000
  ICC/IF 1:50-1:200
  IP 1:50

• Other Gene Names:

  CDK4

• Subcellular Location:

  Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G (1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G (1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.

• Protein Name:

  P2X purinoceptor 1