Anti-IRF7 Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-IRF7 Rabbit Monoclonal Antibody catalog # M00115. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Interferon regulatory factor 7; IRF-7; IRF7

• Gene Name:

  Interferon regulatory factor 7

• UniProt:

  Q92985

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human IRF7

• Clonality:

  Monoclonal

• Clone:

  Clone: FAH-9

• Tissue Specificity:

  Expressed predominantly in spleen, thymus and peripheral blood leukocytes.

• Applications:

  WB, IHC, ICC, IF, IP, Flow Cytometry

• Field of Research:

  Antiviral Signaling, Cytokines, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Host-Virus Interaction, Immune System Diseases, Immunology, Innate Immunity, Interferons, Interspecies Interaction, Microbiology, Pol II Transcription, Polymerase Associated Factors, TLR Signaling, Transcription

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Key transcriptional regulator of type I interferon (IFN) -dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88- independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either ly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  IP 1:50
  FC 1:50

• Other Gene Names:

  IRF7

• Subcellular Location:

  Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus.

• Protein Name:

  P2X purinoceptor 1