Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody catalog # M00086. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Runt-related transcription factor 1; Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; RUNX1; AML1, CBFA2

• Gene Name:

  Runt-related transcription factor 1

• UniProt:

  Q01196/Q13761/Q13950

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human RUNX1/RUNX2/RUNX3

• Clonality:

  Monoclonal

• Clone:

  Clone: DOG-18

• Tissue Specificity:

  Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

• Applications:

  WB, IHC, ICC, IF, IP, Flow Cytometry

• Field of Research:

  Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Organogenesis, Skeletal Development, Stem Cells, Transcription, Transcription Factors

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down- regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532) . .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  IP 1:50
  FC 1:50

• Other Gene Names:

  RUNX1

• Subcellular Location:

  Nucleus.

• Protein Name:

  P2X purinoceptor 1