Anti-LIM domain-binding protein 2 LDB2 Antibody

Anti-LIM domain-binding protein 2 LDB2 Antibody

• Background: LDB2 (LIM homeobox protein cofactor CLIM-1a). It is suggested that LIM domain binding proteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. LDB2 is a close homologue of LDB1. LDBs are homologs of the Drosophila Chip protein. Three members of the ldb gene family (Ldb1, Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95%, 73% and 62% amino acid identity with mouse Ldb1, respectively.
• Description: Boster Bio Anti-LIM domain-binding protein 2 LDB2 Antibody (Catalog # A09871). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
• Synonyms: LDB2, LDB2/CLIM1, CLIM1, LDB-2, CLIM-1, LIM domain-binding protein 2, Carboxyl-terminal LIM domain-binding protein 1, LIM domain-binding factor CLIM1, Nuclear LIM interactor
• CAS Number: 9007-83-4
• Gene Name: LDB2
• UniProt: O55203
• Host: Rabbit
• Reactivity: Human, Mouse
• Cross Reactivity: No cross reactivity with other proteins.
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2.
• Clonality: Polyclonal
• Applications: ELISA, IHC, WB
• Purification: This affinity purified antibody is directed against mouse LDB2. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100% homology for the immunogen sequence. Cross-reactivity with LDB2 homologues from other sources has not been determined.
• Concentration: 1.06 mg/mL by UV absorbance at 280 nm
• Form: Liquid (sterile filtered)
• Molecular Weight: 42793 MW
• Components: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
• Storage Conditions: Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
• Fragment: IgG
• Applications Notes: This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB2 by western blotting in the appropriate cell lysate or extract.