Anti-LIM domain-binding protein 2 LDB2 Antibody

• Background:

  LDB2 (LIM homeobox protein cofactor CLIM-1a) . It is suggested that LIM domain binding proteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. LDB2 is a close homologue of LDB1. LDBs are homologs of the Drosophila Chip protein. Three members of the ldb gene family (Ldb1, Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95%, 73% and 62% amino acid identity with mouse Ldb1, respectively.

• Description:

  Boster Bio Anti-LIM domain-binding protein 2 LDB2 Antibody (Catalog # A09871) . Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

• Synonyms:

  LDB2, LDB2/CLIM1, CLIM1, LDB-2, CLIM-1, LIM domain-binding protein 2, Carboxyl-terminal LIM domain-binding protein 1, LIM domain-binding factor CLIM1, Nuclear LIM interactor

• Gene Name:

  LDB2

• UniProt:

  O55203

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Cross Reactivity:

  No cross reactivity with other proteins.

• Immunogen:

  This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2.

• Clonality:

  Polyclonal

• Applications:

  ELISA, IHC, WB

• Field of Research:

  Epigenetics and Nuclear Signaling, Transcription

• Purification:

  This affinity purified antibody is directed against mouse LDB2. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100% homology for the immunogen sequence. Cross-reactivity with LDB2 homologues from other sources has not been determined.

• Concentration:

  1.06 mg/mL by UV absorbance at 280 nm

• Form:

  Liquid (sterile filtered)

• Function:

  Binds to the LIM domain of a wide variety of LIM domain- containing transcription factors.

• Molecular Weight:

  42793 MW

• Shipping Conditions:

  Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

• Storage Conditions:

  Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

• Fragment:

  IgG

• Applications Notes:

  This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB2 by western blotting in the appropriate cell lysate or extract.

• Other Gene Names:

  LIM domain-binding protein 2

• Subcellular Location:

  Nucleus.

• Prediction Reactivity:

  Chicken, Chimpanzee, Canine