Anti-TUT1/Tutase Antibody

• Background:

  Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
  
  Ota T., Nat. Genet. 36:40-45(2004).
  The MGC Project Team, Genome Res. 14:2121-2127(2004).
  Trippe R., RNA 12:1494-1504(2006).

• Description:

  Boster Bio Anti-TUT1/Tutase Antibody (Catalog # A09330) . Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

• Synonyms:

  Speckle targeted PIP5K1A-regulated poly (A) polymerase; Star-PAP;2.7.7.19; RNA-binding motif protein 21; RNA-binding protein 21; U6 snRNA-specific terminal uridylyltransferase 1; U6-TUTase;2.7.7.52; TUT1; RBM21

• Gene Name:

  TUT1

• UniProt:

  Q9H6E5

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Synthesized peptide derived from internal of human TUT1.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widely expressed. .

• Applications:

  WB, IHC

• Field of Research:

  Chromatin Binding Proteins, DNA/RNA, DNA/RNA Binding, Epigenetics and Nuclear Signaling, RNA Processing

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Form:

  Liquid

• Function:

  Poly (A) polymerase that creates the 3'-poly (A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly (A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication- dependent histone mRNA degradation. .

• Molecular Weight:

  93847 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Other Gene Names:

  Speckle targeted PIP5K1A-regulated poly (A) polymerase

• Subcellular Location:

  Nucleus, nucleolus . Nucleus speckle .