Anti-F-box only protein 4 FBXO4 Antibody

• Background:

  Skp/Cullin/F-box (SCF) complexes are part of a large family of E3 ubiquitin ligases in which F-box proteins determine substrate specificity. Substrate selection by F-box proteins may also be aided by accessory cofactors. The F-box only protein 4, FBX4, interacts via its F-box with SKP1 and CUL1 to form SCFFBX4 ubiquitin ligase. The activity of the SCFFBX4 complex can be regulated by aB crystallin, a small heat shock protein which serves as a specificity cofactor for FBX4 dependent ubiquitination. FBX4 together with aB crystallin (SCFFBX4-aB crystallin) promotes the ubiquitination of cyclin D1 phosphorylated at Thr-286. In addition, the SCFFBX4-aB crystallin ubiquitin ligase has been implicated in the ubiquitination of unknown substrates in desmin-related myopathy. SCFFBX4, in the absence of aB crystallin, also ubiquitinates the telomeric protein PIN2/TRF1.

• Description:

  Boster Bio Anti-F-box only protein 4 FBXO4 Antibody (Catalog # A08044) . Tested in IF, WB applications. This antibody reacts with Human, Mouse.

• Synonyms:

  Anti-F-box only protein 4, Fbx4, FBXO4, F box protein 4, F box protein Fbx4, FBX4, FBXO 4, FLJ10141

• Gene Name:

  FBXO4

• UniProt:

  Q8CHQ0

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide derived from sequences unique to the N-terminus of FBX4 and conserved between the human and murine proteins (see link below for the full-length sequence of the mouse gene product) .

• Clonality:

  Polyclonal

• Applications:

  IF, WB

• Field of Research:

  Cell Biology, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, SCF Complex E3 Ligase, Ubiquitin E3 Enzymes

• Purification:

  This antiserum is directed against human and mouse FBX4. Reactivity against homologues from other sources is not known.

• Concentration:

  85 mg/mL by Refractometry

• Form:

  Liquid (sterile filtered)

• Shipping Conditions:

  Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

• Storage Conditions:

  Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

• Fragment:

  Antiserum

• Applications Notes:

  This antiserum has been tested for use in western blotting and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size by western blotting in the appropriate cell lysate or extract.