Anti-ASPP1 Ppp1r13b Antibody

• Background:

  ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.

• Description:

  Boster Bio Anti-ASPP1 Ppp1r13b Antibody (Catalog # A07257) . Tested in ELISA, WB applications. This antibody reacts with Human.

• Synonyms:

  ASPP1, ASPP-1, ASPP 1, p53BP2 like, p85, PPP1R13B

• Gene Name:

  PPP1R13B

• UniProt:

  Q96KQ4

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP1.

• Clonality:

  Polyclonal

• Applications:

  ELISA, WB

• Field of Research:

  Cancer, Oncoproteins, Oncoproteins/Suppressors, Stem Cells

• Purification:

  This affinity purified antibody is directed against human ASPP1. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Minimal reactivity occurs against ASPP2. A BLAST analysis was used to suggest cross-reactivity with ASPP1 from mouse based on a 92% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

• Concentration:

  0.33 mg/mL by UV absorbance at 280 nm

• Form:

  Liquid (sterile filtered)

• Function:

  Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo (By similarity) .

• Molecular Weight:

  119170 MW

• Shipping Conditions:

  Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

• Storage Conditions:

  Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

• Fragment:

  IgG

• Applications Notes:

  This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to ASPP1 by western blotting in the appropriate cell lysate or extract.

• Other Gene Names:

  Apoptosis-stimulating of p53 protein 1

• Subcellular Location:

  Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity) . Some fraction is nuclear.