Anti-WHIP WRNIP1 Antibody

Anti-WHIP WRNIP1 Antibody

• Background: Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging.  Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process. A second isoform exists (WHIP2).
• Description: Boster Bio Anti-WHIP WRNIP1 Antibody (Catalog # A06521). Tested in ELISA, WB applications. This antibody reacts with Human.
• Synonyms: WHIP, ATPase WRNIP 1, ATPase WRNIP1, Werner helicasae interacting protein 1, WRNIP-1
• CAS Number: 9007-83-4
• Gene Name: WRNIP1
• UniProt: Q96S55
• Host: Rabbit
• Reactivity: Human
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.
• Clonality: Polyclonal
• Applications: ELISA, WB
• Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase. Reactivity is expected against human, mouse, rat and monkey WHIP1 protein.
• Concentration: 1.0mg/ml by UV absorbance at 280 nm
• Form: Liquid (sterile filtered)
• Components: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
• Storage Conditions: Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
• Fragment: IgG
• Applications Notes: This affinity purified antibody has been tested by WB and ELISA. Anti-WHIP is useful in western blotting against HEK293 whole cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 96.0 kDa (predicted molecular weight: 72.2 kDa). Specific band detection by western blot is blocked by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.