Anti-WHIP WRNIP1 Antibody

• Background:

  Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging.  Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process. A second isoform exists (WHIP2).

• Description:

  Boster Bio Anti-WHIP WRNIP1 Antibody (Catalog # A06521). Tested in ELISA, WB applications. This antibody reacts with Human.

• Synonyms:

  WHIP, ATPase WRNIP 1, ATPase WRNIP1, Werner helicasae interacting protein 1, WRNIP-1

• Gene Name:

  WRNIP1

• UniProt:

  Q96S55

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.

• Clonality:

  Polyclonal

• Applications:

  ELISA, WB

• Purification:

  This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase. Reactivity is expected against human, mouse, rat and monkey WHIP1 protein.

• Concentration:

  1.0mg/ml by UV absorbance at 280 nm

• Form:

  Liquid (sterile filtered)

• Components:

  0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

• Storage Conditions:

  Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

• Fragment:

  IgG

• Applications Notes:

  This affinity purified antibody has been tested by WB and ELISA. Anti-WHIP is useful in western blotting against HEK293 whole cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 96.0 kDa (predicted molecular weight: 72.2 kDa). Specific band detection by western blot is blocked by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.

• CAS Number:

  9007-83-4