Anti-p47 ING3 Antibody

• Background:

  P47 ING3 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Over-expression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) that encodes the longest isoform.

• Description:

  Boster Bio Anti-p47 ING3 Antibody (Catalog # A05458) . Tested in ELISA, WB applications. This antibody reacts with Human.

• Synonyms:

  P47 ING3, p47ING3, ING-3, ING 3, Inhibitor of growth family member 3, Inhibitor of growth protein 3

• Gene Name:

  ING3

• UniProt:

  Q9NXR8

• Host:

  Goat

• Reactivity:

  Human

• Immunogen:

  This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 285-310 of Human ING3 protein (Inhibitor of growth family, member 3) .

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in brain, heart, kidney, liver, lung, ovaries, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus.

• Applications:

  ELISA, WB

• Field of Research:

  Acetylation, Cancer Susceptibility, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Transcription, Tumor Suppressors

• Purification:

  This is an affinity-purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p47 ING3 protein. This sequence only shows homology to isoform 1 for ING3. The sequence is not present in isoforms 2 and 3. However, 100% homology is on record for this protein from human, dog, rat, chimpanzee and orangutan. Cross-reactivity with p47 ING3 protein from mouse and chicken sources is also expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p47 ING3 proteins from other sources is not known.

• Concentration:

  1.10 mg/mL by UV absorbance at 280 nm

• Form:

  Liquid (sterile filtered)

• Function:

  Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.

• Molecular Weight:

  46743 MW

• Shipping Conditions:

  Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

• Storage Conditions:

  Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

• Fragment:

  IgG

• Applications Notes:

  This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band at approximately 47 kDa in size corresponding to ING3 isoform 1 by western blotting in the appropriate cell lysate or extract.

• Other Gene Names:

  Inhibitor of growth protein 3

• Subcellular Location:

  Nucleus.