Anti-VRK2 Antibody

• Description:

  Boster Bio Anti-VRK2 Antibody (Catalog# A04821-1) . Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.

• Synonyms:

  VRK2; Serine/threonine-protein kinase VRK2; Vaccinia-related kinase 2

• Gene Name:

  VRK2

• UniProt:

  Q86Y07

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A peptide derived from human VRK2. Immunogen sequence location: 171 - 220

• Clonality:

  Polyclonal

• Tissue Specificity:

  Isoform 1 and isoform 2 are expressed in various tumor cell lines. Expression of isoform 1 inversely correlates with ERBB2 in breast carcinomas (at protein level) . Widely expressed. Highly expressed in fetal liver, skeletal muscle, pancreas, heart, peripheral blood leukocytes and testis.

• Applications:

  WB, ELISA

• Field of Research:

  Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

• Purification:

  The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Function:

  Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant.

• Molecular Weight:

  58141 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Other Gene Names:

  Serine/threonine-protein kinase VRK2

• Subcellular Location:

  Isoform 1: Cytoplasm. Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Mitochondrion membrane; Single-pass type IV membrane protein.