Mouse Gp130/IL6ST PicoKine® Quick ELISA Kit

Mouse Gp130/IL6ST PicoKine® Quick ELISA Kit

• Background: GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes.Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
• Description: Mouse Gp130/IL6ST PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Il6st in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Interleukin-6 receptor subunit beta;IL-6 receptor subunit beta;IL-6R subunit beta;IL-6R-beta;IL-6RB;Interleukin-6 signal transducer;Membrane glycoprotein 130;gp130;Oncostatin-M receptor subunit alpha;CD130;Il6st;
• CAS Number: 7732-18-5
• Gene Name: Interleukin-6 receptor subunit beta
• Gene ID: 16195
• UniProt: Q00560
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q23-E617
• Applications: ELISA
• Sensitivity: <10 pg/ml
• Function: Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development.
• : heparin, EDTA or citrate
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
• Detection Range: 125 pg/ml - 8,000 pg/ml
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 102451 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	159	185	185	168	174	11.21	6.4%
  Sample 2	1404	1352	1232	1273	1315	66.97	5%
  Sample 3	4588	4716	4423	4674	4600	112.25	2.4%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse Gp130