Monkey primate NGF/NGF Beta ELISA Kit PicoKine®

Monkey primate NGF/NGF Beta ELISA Kit PicoKine®

• Background: Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male monkey primate submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is monkey primate 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.
• Description: Monkey primate NGF/NGF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Monkey NGF in cell culture supernatants and serum. Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Beta-nerve growth factor;Beta-NGF;NGF;NGFB;
• CAS Number: 7732-18-5
• Gene Name: Beta-nerve growth factor
• Gene ID: 711681
• UniProt: P01138
• Reactivity: Monkey
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S122-A241
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants and serum.
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <15 pg/ml
• Function: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). .
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 26959 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	50	52	40	60	51	2.29	4.5%
  Sample 2	99	89	107	76	93	3.79	4.1%
  Sample 3	673	660	887	1000	805	33.97	4.2%

  *number of samples for each test n=16.
• Subcellular Location: Secreted.
• Sequence Similarities: Belongs to the NGF-beta family.
• Protein Name: Beta-nerve growth factor