Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®

• Background:

  Plasma kallikrein?is a?protein?that in humans is encoded by the?KLKB1?gene. It is mapped to 4q35.2. This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms.

• Description:

  Mouse KLKB1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Klkb1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  Plasma kallikrein; Fletcher factor; Kininogenin; Plasma prekallikrein; PKK; Plasma kallikrein heavy chain; Plasma kallikrein light chain; KLKB1; KLK3

• Gene Name:

  Klkb1

• UniProt:

  P26262

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: G20-A638

• Applications:

  ELISA

• Sample Type:

  Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <50 pg/ml

• Function:

  The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

• Storage Conditions:

  Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Other Gene Names:

  Klkb1

• Subcellular Location:

  Secreted