Mouse R-Cadherin-4 Cdh4 ELISA Kit PicoKine®

• Background:

  Cadherin-4 is a protein that in humans is encoded by the CDH4 gene. It is mapped to 2 H4; 2 101.86 cM. This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. The encoded protein is involved in retinal angiogenesis during development where it plays a crucial role in the endothelial-astrocyte interactions. Alternative splicing results in multiple transcript variants encoding different isoforms.

• :

  heparin, EDTA or citrate

• Description:

  Mouse R-Cadherin-4 Cdh4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cdh4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	193	219	205	218	208	10.63	5.1%
  Sample 2	781	864	833	852	832	31.72	3.8%
  Sample 3	4510	4030	4318	4174	4258	177.58	4.1%

  *number of samples for each test n=16.

• Synonyms:

  Cadherin-4; Retinal cadherin; R-CAD; R-cadherin; Cdh4

• Gene Name:

  cadherin 4

• Gene ID:

  12561

• UniProt:

  P39038

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: D167-A731

• Tissue Specificity:

  Distributed widely in mouse tissues with high levels present in brain, skeletal muscle and thymus.

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <50 pg/ml

• Function:

  Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Specificity:

  Natural and recombinant mouse Cadherin-4/R-Cadherin

• Subcellular Location:

  Cell membrane. Single-pass type I membrane protein.

• CAS Number:

  7732-18-5