Human Chitotriosidase/CHIT1 ELISA Kit PicoKine®

Human Chitotriosidase/CHIT1 ELISA Kit PicoKine®

• Background: Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1q32.1. Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.
• Description: Human Chitotriosidase/CHIT1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CHIT1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Chitotriosidase-1; Chitinase-1; CHIT1
• CAS Number: 7732-18-5
• Gene Name: chitinase 1
• Gene ID: 1118
• UniProt: Q13231
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A22-N466
• Applications: ELISA
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA).
• Detection Range: 312 pg/ml - 20,000 pg/ml
• Sensitivity: <50 pg/ml
• : heparin or EDTA
• Function: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	590	572	610	690	615	45.06	7.3%
  Sample 2	1670	1703	1941	1771	1771	104.55	5.9%
  Sample 3	7933	8323	9098	7778	8283	510.72	6.1%

  *number of samples for each test n=16.
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant human CHIT1