Human CPE/Carboxypeptidase E ELISA Kit PicoKine®

Human CPE/Carboxypeptidase E ELISA Kit PicoKine®

• Background: This gene encodes a member of the M14 family of metallocarboxypeptidases. It is mapped to 4q32.3. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.
• Description: Human CPE ELISA Kit PicoKine® (96 Tests). Quantitate Human CPE in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase
• CAS Number: 7732-18-5
• Gene Name: carboxypeptidase E
• Gene ID: 1363
• UniProt: P16870
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: L43-F476
• Applications: ELISA
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA).
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <50 pg/ml
• : heparin or EDTA
• Function: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	55	51	51	51	52	1.73	3.3%
  Sample 2	259	231	228	247	241	12.53	5.1%
  Sample 3	792	756	822	884	813	46.93	5.7%

  *number of samples for each test n=16.
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant human CPE