Human Syndecan-4/SDC4 ELISA Kit PicoKine®

Human Syndecan-4/SDC4 ELISA Kit PicoKine®

• Background: Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.
• Description: Human Syndecan-4/SDC4 ELISA Kit PicoKine® (96 Tests). Quantitate Human SDC4 in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Syndecan-4;SYND4;Amphiglycan;Ryudocan core protein;SDC4;
• CAS Number: 7732-18-5
• Gene Name: Syndecan-4
• Gene ID: 6385
• UniProt: P31431
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: E19-E145
• Applications: ELISA
• Sample Type: cell culture supernatants, cell lysates and serum.
• Detection Range: 62.5 pg/ml - 4,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Cell surface proteoglycan that bears heparan sulfate.
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 21642 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	94	82	81	78	83	6.09	7.3%
  Sample 2	673	735	621	630	664	45.06	6.7%
  Sample 3	2108	2092	1869	1903	1993	107.82	5.4%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant human Syndecan-4