Human CCL16/HCC-4 ELISA Kit PicoKine®

• Background:

  Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1) . This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.

• Description:

  Human CCL16/HCC-4 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CCL16 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  C-C motif chemokine 16; Chemokine CC-4; HCC-4; Chemokine LEC; IL-10-inducible chemokine; LCC-1; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant; LMC; Monotactin-1; MTN-1; NCC-4; Small-inducible cytokine A16; CCL16; ILINCK, NCC4, SCYA16

• Gene Name:

  C-C motif chemokine 16

• UniProt:

  O15467

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: Q24-Q120

• Tissue Specificity:

  Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

• Applications:

  ELISA

• Field of Research:

  Angiogenesis, Cardiovascular, Chemokines, Cytokines, Immunology, Innate Immunity, Macrophage/Inflammation

• Sample Type:

  Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

• Detection Range:

  7.8 pg/ml - 500 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.

• Storage Conditions:

  Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Other Gene Names:

  CCL16

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the intercrine beta (chemokine CC) family.

• Protein Name:

  C-C motif chemokine 16